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Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf
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Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf
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Can the protozoan parasite Bonamia ostreae infect larvae of flat oysters Ostrea edulis? ArchiMer
Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine.
Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled...
Tipo: Text Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf
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Bonamia ostreae and Ostrea edulis : a stable host-parasite system in France? ArchiMer
Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Francois, Cyrille; Joly, Jean-pierre.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. After its first description in June 1979 in L'Ile Tudy, Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. Bonamiosis and marteiliosis, another protozoan disease appeared in the seventies, drastically reduced the French flat oyster production from 20 000 tonnes in 1970 to less than 2000 tonnes after 1981. In 2001, about 1650 t of flat oysters were marketed in France. The production is restricted to several areas specialised in reproduction, growth and/or marketing, and depends on transfers of animals. Most important production...
Tipo: Text Palavras-chave: Bonamia ostreae; Parasitology; Pathology; Ostrea edulis; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-6381.pdf
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A long term study of bonamiosis in Quiberon bay, France ArchiMer
Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck.
Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the...
Tipo: Text Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf
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Stratégie d'utilisation de différentes espèces animales par le parasite Marteilia refringens pour assurer son cycle biologique ArchiMer
Audemard, Corinne.
Au cours des trente dernières années, la production française d 'huître plate Ostrea edulis est passée de 30 000 tonnes par an à seulement 1 500 tonnes actuellement. Le parasite de la glande digestive de l'huître plate, Marteilia refringens, est en partie responsable de cette chute de production. La gestion des risques liés au développement de cette maladie en zone endémique, nécessite la mise en évidence des compartiments fonctionnels de ce cycle parasitaire. Les travaux antérieurs ont montré que le cycle de M. refringens était vraisemblablement hétéroxène, cependant cette hypothèse n'avait jamais pu être démontrée, limitant cette gestion. La recherche d'hôtes de M. refringens a pu être réalisée par l'application d'outils de biologie moléculaire (PCR et...
Tipo: Text Palavras-chave: Pathologie des mollusques; Marteilia refringens; Ostrea edulis; Paracartia grani; Cycle de vie; Outils de biologie moléculaire; Modèle d'étude.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00107/21850/19440.pdf
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Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens ArchiMer
Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck.
Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of...
Tipo: Text Palavras-chave: Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-2778.pdf
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Infection dynamics of Marteilia refringens in flat oyster Ostrea edulis and copepod Paracartia grani in a claire pond of Marennes-Oleron Bay ArchiMer
Audemard, Corinne; Sajus, Marie-céline; Barnaud, Antoine; Sautour, Benoît; Sauriau, Pierre-guy; Berthe, Franck.
The protozoan parasite Marteilia refringens has been partly responsible for the severe decrease in the production of the European flat oyster Ostrea edulis Linnaeus in France since the 1970s. The calanoid copepod Paracartia grani Sars was recently found to be a host for M refringens in French shallow-water oyster ponds ('claires'). This study reconsidered M refringens transmission dynamics in the light of this finding, taking into account not only oyster infection dynamics and environmental factors but also data concerning the copepod host. P. grani population dynamics in the claire under study revealed that this species is the dominant planktonic copepod in this confined ecosystem. During winter, M refringens overwintered in O. edulis, with P. grani...
Tipo: Text Palavras-chave: Claire pond; Infection dynamics; Paracartia grani; Ostrea edulis; Marteilia refringens.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2858.pdf
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Bonamia ostreae (Pichot et Coll., 1979) parasite de l'huître plate : comparaison de deux méthodes de diagnostic ArchiMer
Bachere, Evelyne; Durand, Jean-louis; Tige, Gilbert.
The parasite, Bonamia ostreae, was diagnosed in several South Brittany flat oyster's samples, from gills tissues's prints, stained in May-Grunwald-Giemsa and from corresponding digestive glands sections. The comparaison of results shows quantitative differences relating to the infested oyster's rate, and qualitative differences relating to the early stages of infestation. Gill's print technic seems more intersting for the only Bonamia ostreae diagnosis.
Tipo: Text Palavras-chave: Pathologie; Parasite; Huître plate; Ostrea edulis; Bonamia ostreae.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/acte-5013.PDF
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Mise en évidence d'Haplosporidium sp. (Haplosporida - Haplosporidiidae) parasite de l'huître plate Ostrea edulis L. ArchiMer
Bachere, Evelyne; Grizel, Henri.
A new Haplosporidie has been observed in the flat oyster Ostrea edulis from the Mediterranean Sea. Different development stages have been described in light and electron microscopy. The characteristics of the spores and especially the presence of at least four sporal filaments enable us to distinguish this Haplosporidie from those already described in the Atlantic flat oysters. Different haplosporidies have been described in the oyster. Some are known as being pathogenic, Minchinia costalis (Andrews et al., 1962 and 1978; Couch 1967) and Minchinia nelsoni (Haskin et al., 1966; Farley 1968 and 1975), others, identified only recently, are considered as occasional parasites (Van Banning, 1977). During the research conducted on Bonamia ostreae (Pichot et al.,...
Tipo: Text Palavras-chave: Pathologie; Haplosporidium; Parasite; Ostrea edulis; Huître plate.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-6576.PDF
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Studies of a European shellfish disease: Bonamia ostreae parasite of the flat oyster Ostrea edulis ; control and treatment. Final Report ArchiMer
Bachere, Evelyne; Ifremer, Unité de Pathologie, Immunologie et Génétique Moléculaire des Invertébrés Marins, F-17390 La Tremblade, France; CEV, Centro Experimental de Vilaxoan, Conselleria de Pesca, Villagarcia de Arosa, Spain; CSIC, Consejo Superior Investigaciones Cientificas, Peira de Bouzas S/N, Avda E. Cabello 636208, Vigo, Spain; UCC, University College Cork, Dept of Zoology Cork, Ireland; FCR, Fisheries Research Center, Abbotstown, Castleknock, Dublin 15, Ireland; US, University of Southampton, Dept of Oceanography Southampton S09 5NH, United Kingdom.
Bonamia ostreae, an intrahemocytic protozoan parasite infecting the oyster Ostrea edulis, is responsible for an endemic disease affecting all intensive breeding areas of European countries. Epidemiological studies showed variable prevalences, 43% in Galicia (Spain), from 6% to 32% in France and 45% in the most important area of Ireland. The impact of oyster transfers, handlings, high densities have been implicated in the disease development but environmental stressors could be more involved than zootechnical methods. The effect of water temperature, salinity and tidal exposure were particulary examined to assess stress induced changes and determine physiological, biochemical and immunological state indices of oyster populations, which will contribute to...
Tipo: Text Palavras-chave: Disease; Oyster; Ostrea edulis; Parasitology; Bonamia ostreae; Epidemiology.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00273/38383/36713.pdf
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Etudes sur la pathologie des huîtres ArchiMer
Balouet, G..
French summary: In 1974, at the request of a group of oyster farmers in the Brittany region, the Pathology Laboratory of the U.E.R. Medicine of Brest undertook a series of theoretical and applied research projects concerning the problem of flat oyster digestive gland disease, due to Marteilia refringens, the primary economic problem of that time. In 1978, in the research report of Contract C.N.E.X.O. 77/1690, the main results obtained since the beginning of the study of the parasitosis were made public. The present report has as its objective to show: - The results obtained in 1978-1979 while monitoring the parasitosis, and most especially the epidemiological evolution of the disease; - Information concerning the presence of other infectious diseases...
Tipo: Text Palavras-chave: Parasite; Ostrea edulis; Oyster; Pathology.
Ano: 1978 URL: http://archimer.ifremer.fr/doc/1978/rapport-1927.pdf
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Nursery function of coastal temperate benthic habitats: New insight from the bivalve recruitment perspective ArchiMer
Barbier, Pierrick; Meziane, Tarik; Foret, Martin; Tremblay, Rejean; Robert, Rene; Olivier, Frederic.
Marine habitat function has been typically investigated in terms of biogeochemical regulation but rarely in terms of population renewal, which is mainly controlled by recruitment dynamics. The recruitment phase is crucial for organisms with a bentho-pelagic life cycle, such as bivalves, and it regulates the population renewal success. This study provides new insight on the role of temperate benthic habitats on bivalve recruitment, as a function of nursery areas. Six dominant benthic habitats of the Chausey archipelago (Normandy, France) were studied. In each habitat, bivalve recruit assemblages were described at the end of two reproductive seasons. Furthermore, Ostrea edulis juveniles were immerged on each habitat during two months to compare growth...
Tipo: Text Palavras-chave: Benthic habitats; Bivalve recruitment; Nursery function; Ostrea edulis; Fatty acids.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00367/47863/47845.pdf
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Etude de la dynamique du parasite Marteilia refringens chez son hôte Ostrea edulis ArchiMer
Barnaud, Antoine.
Après une synthèse des principaux agents pathogènes en conchyliculture, l'auteur présente le protozoaire Marteilia refringens, parasite majeur de l'huître plate Ostrea edulis en Europe, dont le cycle reste inconnu. Suit une étude expérimentale de la dynamique du parasite dans l'huître, intégrée à un modèle d'étude de la marteiliose en claire ostrécole, montrant l'existence d'une fenêtre temporelle d'infection et aboutissant à une nouvelle hypothèse de cycle parasitaire. Ces résultats sont enfin mis en relation avec la détection récente de Marteilia refringens dans le copépode Acartia grani, hôte intermédiaire potentiel.
Tipo: Text Palavras-chave: Marteilia refringens; Ostrea edulis; Huître; Conchyliculture; Pathologie; Paramyxea.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00109/22038/19683.pdf
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Historique, expériences en cours et perspectives du programme de sélection de l'huître plate Ostrea edulis pour la résistance à la bonamiose ArchiMer
Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors.
Tipo: Text Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf
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Comparaison de la croissance et de la mortalité de différentes familles et lignées d'huître plate Ostrea edulis en système intensif ArchiMer
Baud, Jean-pierre; Le Bayon, Nicolas; Haure, Joel; Nourry, Max; Palvadeau, Hubert; Penisson, Christian; Naciri-graven, Yamama; Goyard, Emmanuel; Barre, Marc.
As of 1985, Ifremer set up a selection programme for resistance to the protozoan Bonamia ostreae in European flat oysters (Ostrea edulis). Two selection lines, L85 and L89, from the third and second generation, respectively, were produced with 17 and 21 different families. The experimental design also included a mixed line (L85 x L89) of seven families and a control line of five families from Quiberon broodstock maintained without selection pressure.
Tipo: Text Palavras-chave: Croissance; Génétique; Huîtres plates; Ostrea edulis.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3245.pdf
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L'amélioration des performances de l'huître plate européenne Ostrea edulis par la sélection génétique ArchiMer
Bedier, Edouard; Baud, Jean-pierre; Cochennec, Nathalie; Cornette, Florence; Gerard, Andre; Goyard, Emmanuel; Joly, Jean-pierre; Kopp, Joel; Langlade, Aime; Le Coguic, Yvette; Mazurie, Joseph; Nourry, Max.
The European flat oyster Ostrea edulis has been considered as the traditional French oyster for a very long time and has now nearly disappeared from our farms, as a consequence of the two successive epizootic of the Marteilia refringens parasite and Bonamia ostreae. The actual quasi monoculture of Crassostrea gigas has made necessary the search for a diversification in oyster culture, including the search for a new start of the indigenous flat oyster culture. The Ifremer has been conducting parallel works since 1985 in order to better understand the biology of the parasite as well as to try and select different lines of flat oyster resistant to Bonamia ostreae.
Tipo: Text Palavras-chave: Bonamia ostrea; Huître plate; Sélection Génétique; Ostrea edulis.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3274.pdf
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Validation in commercial conditions of the response to selection of the European flat oyster Ostrea edulis for resistance to Bonamia ostreae ArchiMer
Bedier, Edouard; Langlade, Aime; Angeri, Stéphane; Brizard, Raphael; Nerlovic, V.; Glize, Philippe; Haffray, Philippe.
Since the outbreaks of the parasitosis with Marteilia refringens and Bonamia ostreae in the last 60's, the French production of the European flat oyster dropped from 20000 mT to less than 1500 mT currently, leaving the French shellfish farming in situation of virtually monoculture of the Pacific cupped oyster Crassostrea gigas. To sustain this traditional culture, Ifremer has developed, at the experimental level, a genetic selection for a better tolerance to Bonamia ostreae which led to an improvement of survivals. The transfer of these results to the business scale requires the control of the reproduction in hatchery and the adaptation of the cycle of production to the constraints of seedlings in deep water as practised in Brittany (France). That's why a...
Tipo: Text Palavras-chave: Selection; Genetic; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3313.pdf
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Taxinomie et épidémiologie moléculaires du parasite Marteilia refringens Grizel et al. 1974 : intérêt pour la gestion du risque sanitaire en conchyliculture ArchiMer
Berthe, Franck.
Infectious diseases are currently recognised as a major limiting factor to development and sustainability of aquaculture of molluscs. The study is focused on the European flat oyster, Ostrea edulis and its paramyxean parasite Marteilia refringens. Results are contributing to the prevention of risk associated to infected stocks imports from endemic zones, by developing molecular diagnostic tools that are specific, sensible and reliable. Phylogeny and taxonomy of Marteilia refringens, previously impeded by microscopy limits, were clarified by sequencing the rDNA SSU, small sub-unit in the rRNA gene cluster. These new insights have practical implications discussed in a pragmatic polyphasic approach involving pathology and epidemiology. This approach led us to...
Tipo: Text Palavras-chave: Marteilia refringens; Marteilia maurini; Ostrea edulis; Mytilus edulis; Pathologie; Phylogénie moléculaire; Taxinomie; Epidémiologie; Marteilia refringens; Marteilia maurini; Ostrea edulis; Mytilus edulis; Molluscs diseases; Molecular phylogeny; Taxonomy; Epidemiology.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00109/21980/19594.pdf
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Que savons-nous des Marteillia? ArchiMer
Berthe, Franck; Haffner, Philippe; Zerabib, Maamar; Rodriguez, David.
Marteilia refringens, a parasitic protist, is partially responsible for the drastic reduction in French production of the European flat oyster (Ostrea edulis) in the 1970s and still constitutes a major obstacle for revitalizing coastal farming of this species.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Protiste; Parasite; Marteilia refringens; Pathologie.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3247.pdf
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